FastQCFastQC Report
Sun 14 May 2023
H52YHDSX7_l04_n02_HMGB15-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH52YHDSX7_l04_n02_HMGB15-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36533339
Sequences flagged as poor quality0
Sequence length101
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16590364.541156229930147No Hit
TTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8656122.369375544896129No Hit
TTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3792171.038002576222228No Hit
TTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3598760.9850618910031738No Hit
GTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2329330.6375902295708585No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2203490.6031449794391911No Hit
TTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1673010.457940622399721No Hit
GTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1057960.2895875463230996No Hit
GTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT909530.24895890298995116No Hit
TTTTTTTTTTTTTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT905770.24792970606929743No Hit
TTTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTT872660.23886675127066814No Hit
TTTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTT721580.19751274308652705No Hit
TTTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTTC656810.1797837312379249No Hit
TTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT629540.17231931633733233No Hit
TTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT612570.16767424406512638No Hit
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCG566520.1550693190129706No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT527590.14441329876801023No Hit
GTTTTTTTTTTTTTTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT491750.13460308131156587No Hit
TTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA475160.13006202362176641No Hit
TTTTTTTTTTTTTTTTTTTGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT473240.12953647625802833No Hit
TTTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAA471720.12912041792840231No Hit
TTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTTCG453260.1240674990041288No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGG421700.11542881421268392No Hit
TTTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA401050.10977644282664663No Hit
TTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAAA393570.10772899788875033No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)