FastQCFastQC Report
Sun 14 May 2023
H52YHDSX7_l03_n02_3R_1-10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH52YHDSX7_l03_n02_3R_1-10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10979355
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1717191.5640171940883594No Hit
TTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT475130.4327485539906488No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT473390.43116376144135976No Hit
TTTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198880.18113996678311248No Hit
TTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197290.17969179428117588No Hit
TTTTTTTTTTTTTTTTTTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT157290.14325978165384032Illumina Single End PCR Primer 1 (100% over 31bp)
GTTTTTTTTTTTTTTTTTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118310.1077567853485018No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT113520.10339405183637837No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)