FastQCFastQC Report
Sun 14 May 2023
H52YHDSX7_l02_n02_13R_1-12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH52YHDSX7_l02_n02_13R_1-12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21228496
Sequences flagged as poor quality0
Sequence length101
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC2571451.2113199163991646No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA2291441.0794170251156747No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2209361.0407520155926262No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA2092440.9856751038792385No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT905530.4265634268202514No Hit
TTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCAT888720.41864482533289216No Hit
TTTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATG805420.37940511659422316No Hit
TTTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTC765730.36070854948932796No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA755450.3558660020003301No Hit
TTTTTTTTTTTTTTTTTTCATAAATAACGTCATGCATTACATGTTAATTA422250.19890716704565412No Hit
TTTTTTTTTTTTTTTTTCATAAATAACGTCATGCATTACATGTTAATTAT314320.1480651290604855No Hit
TTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTAC277360.13065456921677354No Hit
GTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA253080.11921711269606665No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)