FastQCFastQC Report
Sun 14 May 2023
H52YHDSX7_l01_n02_7R_1-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH52YHDSX7_l01_n02_7R_1-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18943720
Sequences flagged as poor quality0
Sequence length101
%GC26

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA1705430.9002614058907121No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC1561950.824521266150471No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1480170.7813512868644595No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA1313990.6936282842018358No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT653830.3451434037242949No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA610030.3220222849577591No Hit
TTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCAT539130.2845956338037091No Hit
TTTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTC515000.2718579033051587No Hit
TTTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATG461850.24380111192521847No Hit
TTTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGCA449260.23715510997839917No Hit
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCG309360.16330477857569686No Hit
TTTTTTTTTTTTTTTTTTGATCATAAAAACCCATCTCATAAATAACGTCA273070.1441480342826013No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGG250030.13198569235609478No Hit
TTTTTTTTTTTTTTTTTTCATAAATAACGTCATGCATTACATGTTAATTA239620.12649046755336332No Hit
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGG212540.11219549275432704No Hit
TTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTAC210570.11115557028925681No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)