FastQCFastQC Report
Sun 14 May 2023
H52YHDSX7_l01_n02_4R_1-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH52YHDSX7_l01_n02_4R_1-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26018515
Sequences flagged as poor quality0
Sequence length101
%GC27

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3156121.2130284914415752No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA1001750.3850142869414338No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC883310.33949285729796647No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA745340.2864652344686082No Hit
TTTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAA671370.2580354797343353No Hit
TTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAAA470310.18075973974686874No Hit
TTTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTTC430090.1653015170158635No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT363110.13955831068760075No Hit
TTTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA349270.13423902171203853No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA316720.12172869973555371No Hit
TTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCAT294350.1131309761529434No Hit
TTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTTCG276270.1061820784160818No Hit
TTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAA266350.10236940886134355No Hit
TTTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTC264590.1016929674887287No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG262140.10075133035071372No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)