FastQCFastQC Report
Sun 14 May 2023
H52YHDSX7_l01_n02_4G_1-14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH52YHDSX7_l01_n02_4G_1-14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20241876
Sequences flagged as poor quality0
Sequence length101
%GC30

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6271713.098383766405841No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA1003100.49555683475187773No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC900380.4448105501683737No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA750120.3705783001536024No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG547110.2702862126020335No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA469070.23173247380825768No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT362930.17929662250672812No Hit
TTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCAT296470.14646369733714404No Hit
TTTTTTTTTTTTTTTTTTGATTAATGAAAACATCCTTGGCAAATGCTTTC267970.1323839746869312No Hit
TTTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTC266110.13146508752449626No Hit
TTTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATG253760.12536387437607066No Hit
TTTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAA241980.11954425568064936No Hit
TTTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGCA206900.10221384618698386No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)