FastQCFastQC Report
Sun 14 May 2023
H52YHDSX7_l01_n02_2G_1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH52YHDSX7_l01_n02_2G_1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24056196
Sequences flagged as poor quality0
Sequence length101
%GC31

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6638002.7593722631791No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA1042660.43342679781957216No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC954210.39665872359869364No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA796300.33101659131809535No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG620390.2578919792638869No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA428670.17819525580852436No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT383080.159243797315253No Hit
TTTTTTTTTTTTTTTTTTGTTGTTGTTGTTGCTTCTCTGCAAATTGCGTT341510.14196342597142125No Hit
TTTTTTTTTTTTTTTTTTGGCTACTAAGATGTTTCAGTTCGCCAGGTTGT341440.14193432743896833No Hit
TTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCAT308700.12832452811741307No Hit
TTTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTC288590.11996493543700758No Hit
TTTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATG274980.11430734934151685No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)