FastQCFastQC Report
Sun 14 May 2023
H52YHDSX7_l01_n02_15R_1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH52YHDSX7_l01_n02_15R_1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16344665
Sequences flagged as poor quality0
Sequence length101
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3827312.3416264573180303No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA682590.41762250862896244No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC605840.3706652904785751No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA479480.2933556607002958No Hit
TTTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAA422490.25848801428478346No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG358930.21960070763151154No Hit
TTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAAA278120.17015949852750117No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA235960.14436514911746434No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT231810.14182609432496782No Hit
TTTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCA229650.14050456219200577No Hit
TTTTTTTTTTTTTTTTTTGCCTTGCGACCATACTCCCCCCGGAACCCAAA198370.12136681908133326No Hit
TTTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTC183090.11201820288149068No Hit
TTTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATG178210.10903251917368756No Hit
TTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCAT177990.10889791867866364No Hit
TTTTTTTTTTTTTTTTTCAGCCTTGCGACCATACTCCCCCCGGAACCCAA166510.10187422012014319No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)