FastQCFastQC Report
Sun 14 May 2023
H52YHDSX7_l01_n02_14G_1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH52YHDSX7_l01_n02_14G_1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27484958
Sequences flagged as poor quality0
Sequence length101
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5088131.8512416864526409No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA1881640.6846071949609673No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC1592770.5795060701930125No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA1360540.4950125810634311No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA820480.2985196484564393No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT684900.24919084831783261No Hit
TTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCAT538370.1958780508232903No Hit
TTTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTC503760.1832857084955342No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG451060.1641115842345475No Hit
TTTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATG450380.163864176179567No Hit
TTTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGCA308090.11209404067490299No Hit
TTTTTTTTTTTTTTTTTTCATAAATAACGTCATGCATTACATGTTAATTA293220.10668380864907999No Hit
GTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA290100.10554864227916957No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)