FastQCFastQC Report
Sun 14 May 2023
H52YHDSX7_l01_n02_12G_1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH52YHDSX7_l01_n02_12G_1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20991372
Sequences flagged as poor quality0
Sequence length101
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3991611.9015479312166923No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA985610.469531005405459No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC860700.4100256048056316No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA716360.3412640202841434No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA392490.18697682076235894No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG366440.174566960177734No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT351950.16766412409822473No Hit
TTTTTTTTTTTTTTTTTTGTTGTTGTTGTTGCTTCTCTGCAAATTGCGTT292040.13912382668460166No Hit
TTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCAT279640.1332166377690796No Hit
TTTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTC266450.12693310375329445No Hit
TTTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATG253010.12053047318679312No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)