FastQCFastQC Report
Sun 14 May 2023
H52YHDSX7_l01_n02_11R_1-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH52YHDSX7_l01_n02_11R_1-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20138933
Sequences flagged as poor quality0
Sequence length101
%GC32

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6031812.9950990948726033No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA2091571.0385704148278363No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC1970950.9786764770506958No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA1671700.8300836990718427No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT828390.411337581787476No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA721350.35818680165428823No Hit
TTTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTC692950.34408476357709716No Hit
TTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCAT683820.3395512562656621No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG634420.315021654821534No Hit
TTTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATG617130.30643629431608915No Hit
TTTTTTTTTTTTTTTTTTCATAAATAACGTCATGCATTACATGTTAATTA325280.16151799104748996No Hit
TTTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGCA265690.13192853861721474No Hit
TTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTAC259830.12901875188720274No Hit
TTTTTTTTTTTTTTTTTCATAAATAACGTCATGCATTACATGTTAATTAT250870.12456965818397629No Hit
TTTTTTTTTTTTTTTTTTGATCATAAAAACCCATCTCATAAATAACGTCA220800.10963838054379545No Hit
TTTTTTTTTTTTTTTTTTGTTTCGTTTAGTACGAGATGGCTTCACACCTC203820.10120695073567204No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)