FastQCFastQC Report
Sun 14 May 2023
H52YHDSX7_l01_n02_11R_1-16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH52YHDSX7_l01_n02_11R_1-16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23815352
Sequences flagged as poor quality0
Sequence length101
%GC28

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2936651.233091159013732No Hit
TTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCA2275850.9556230787602888No Hit
TTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGC2081830.8741546209352689No Hit
TTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCA1754180.7365752981522171No Hit
TTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTCAT872850.3665072848807777No Hit
TTTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTA778510.3268941815346672No Hit
TTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATGCAT702860.2951289571533522No Hit
TTTTTTTTTTTTTTTTTTTAATCATAAAAACCCATCTCATAAATAACGTC694510.2916228154007549No Hit
TTTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGCA689450.28949813548840264No Hit
TTTTTTTTTTTTTTTTTTTCATAAAAACCCATCTCATAAATAACGTCATG632110.26542122912984867No Hit
TTTTTTTTTTTTTTTTTTGATCATAAAAACCCATCTCATAAATAACGTCA480680.20183619372915418No Hit
TTTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCG444570.18667370526373073No Hit
TTTTTTTTTTTTTTTTTCATCTTTACCTCGCGGTACTTGTTCGCTATCGG353450.14841267095275348No Hit
TTTTTTTTTTTTTTTTTTAGCCTTGCGACCATACTCCCCCCGGAACCCAA337260.14161453502765778No Hit
TTTTTTTTTTTTTTTTTTCATAAATAACGTCATGCATTACATGTTAATTA301550.1266200054485863No Hit
TTTTTTTTTTTTTTTTTTATCTTTACCTCGCGGTACTTGTTCGCTATCGG290070.12179958541028493No Hit
TTTTTTTTTTTTTTTTTAAATAACGTCATGCATTACATGTTAATTATTAC271420.11396850233412463No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG267120.11216294430584103No Hit
TTTTTTTTTTTTTTTTTATAAAAACCCATCTCATAAATAACGTCATGCAT255830.10742230473855688No Hit
TTTTTTTTTTTTTTTTTGATCATAAAAACCCATCTCATAAATAACGTCAT247580.10395815270754763No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)