FastQCFastQC Report
Mon 15 May 2023
H52VTDRX3_n02_S3_PBMC_MDCK_TCell.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH52VTDRX3_n02_S3_PBMC_MDCK_TCell.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89682521
Sequences flagged as poor quality0
Sequence length90
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC47647915.3129539032472115No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC18445652.056772021384189No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC10980451.2243690161207668No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC4415380.4923345096420739No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG1673410.18659265833974492No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1216160.13560724948844827Clontech SMART CDS Primer II A (100% over 26bp)
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGTAAA1149360.12815875236156665No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGGAGA992870.11070942129291839No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT953900.10636409295407742No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)