FastQCFastQC Report
Mon 15 May 2023
H52VTDRX3_n02_S3_PBMC_MDCK_BCell.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH52VTDRX3_n02_S3_PBMC_MDCK_BCell.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86286122
Sequences flagged as poor quality0
Sequence length90
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19243932.2302462497966937Clontech SMART CDS Primer II A (100% over 26bp)
TAGCTGCTGGCCGCGCTGCTGCTGATGGGTCGGACCCGGTGGCTGGGCGC5377670.6232369557644507No Hit
GCGTTATCCACCTTCCACTGTACTTTGGCCTCTCTGGGATAGAAGTTATT1754840.20337453571038921No Hit
GGGAAGTTTCTGGCGGTCATTCAGGTCCTGCATGGTCTCCTTCTCGCCCT1467330.1700539977912091No Hit
CACGCTGCTCGTATCCGACGGGGAATTCTCACAGGAGACGAGGGGGAAAA1094340.1268268841656831No Hit
AGCTGCTGGCCGCGCTGCTGCTGATGGGTCGGACCCGGTGGCTGGGCGCC1043140.12089313736918204No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)