FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_wt-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_wt-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11403736
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4695564.117562875885588No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC540020.4735465640383117Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG39300.050.66908318
GAGCACA76450.046.92331716
AGAGCAC78550.046.02669515
AGCACAC52000.042.53557217
CACACGA30800.039.994319
ACACGAG31450.038.94431320
CACGAGA35800.034.5030821
AAGAGCA156400.030.79385614
CACACGT26750.029.30423419
CACGTAG24700.027.90843221
GCACAAG48750.026.63315418
AAGGGGG101450.026.49203767
AAAGGGG105100.025.63949266
CCGTATC185750.025.09445449
CGCCGTA194650.024.5573547
ACACGTA29150.024.3699220
ACGAGAT52000.024.09007822
GCCGTAT207050.023.8809848
CGTATCA198500.023.65878550
CGAGATC52450.023.48301323
Produced by FastQC (version 0.11.2)