FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_wt-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_wt-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11278287
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4268033.7842892276105404No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC352000.3121041342537213Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG45500.051.30192618
GAGCACA74100.045.9096416
AGAGCAC78400.044.51004415
AGCACAC59450.042.56228617
ACACGAG29350.039.10696820
CACACGA29900.038.1547219
CACACGT32350.037.10408819
CACGAGA33850.033.59544421
CACGTAG27350.033.26378621
AAGAGCA151850.029.02142714
ACACGTA33650.027.2461220
CAGATCG108750.026.5736066
CGAGATC47750.024.91468623
ACGAGAT51250.022.59903122
TAGATCG111450.021.4981866
CGTAGAT43300.021.49530623
CGCCGTA145400.021.49012247
CCGTATC143200.021.26025849
GCCGTAT155500.020.74676748
GCACAAG39150.020.20207218
Produced by FastQC (version 0.11.2)