FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_triple-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_triple-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10119071
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3758743.7145109467064716No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1154761.1411719514568086Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG86250.057.5737518
GAGCACA151050.056.9037716
AGAGCAC154950.055.90316415
ACACGAG54800.053.83056620
AGCACAC103650.052.9756617
CACACGA56200.052.4901119
AAGAGCA240000.047.10460314
CACGAGA62850.046.7107821
CACGTAG41350.044.1728521
CACACGT47950.042.61970519
GCACAAG70450.041.0795518
ACACGTA46050.039.5144220
ACGAGAT77700.037.738422
GAGCAAG71050.033.7904116
CAGATCG214400.033.7676856
AGCACAA85850.033.63073317
GAGAGAT121550.032.42028816
AGAGAGA124450.032.08806215
AAGGGGG171200.031.78635667
AAAGGGG172050.031.752366
Produced by FastQC (version 0.11.2)