FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_triple-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_triple-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11343350
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3131152.76033975853694No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC453510.3998025274720431Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG53100.049.2969218
GAGCACA89450.044.68163716
AGAGCAC91100.044.5658415
AGCACAC67700.042.13034417
ACACGAG35900.039.18530720
CACACGA41650.034.44802519
CACACGT34850.034.14063619
CACGAGA42300.033.50119821
AAGAGCA171350.030.82411214
CACGTAG30900.030.68714521
ACACGTA36600.025.43268420
ACGAGAT62700.022.8244122
AAAGGGG94250.021.64787366
CGCCGTA179950.021.39050347
AAGGGGG95250.021.38291267
GCACAAG46600.020.87716918
AGGGGGG102500.020.76098468
CCGTATC177300.020.72448349
GCCGTAT192250.020.42239448
TCGCCGT192900.020.04527946
Produced by FastQC (version 0.11.2)