FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_rad27-exoi-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_rad27-exoi-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7761402
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2066832.6629596044632144No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC311930.40189903834384566Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG50700.057.15281718
GAGCACA87850.053.42323716
AGAGCAC89450.053.1365615
AGCACAC62750.049.74886317
CACACGA35250.048.04873319
ACACGAG35650.046.91913620
CACGAGA39050.043.2803721
CACACGT29100.041.60818519
AAGAGCA142450.041.60003314
CACGTAG24450.040.5016221
CAGATCG86800.037.0455256
ACGAGAT47100.035.88319422
CGAGATC47650.035.7625223
ACACGTA29750.033.64036620
GCACAAG44300.033.02081718
TAGATCG98350.029.6320536
ACGTAGA35950.027.25360722
GCAGATC39850.027.1662565
CGTAGAT36750.026.4697323
AGAGATC71950.026.46062717
Produced by FastQC (version 0.11.2)