FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_rad27-exoi-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_rad27-exoi-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11785366
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3454272.9309823725457487No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC341210.28952007090827725Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG39400.045.11886618
GAGCACA70200.039.6817316
AGAGCAC72950.038.33138715
AGCACAC55950.035.589317
ACACGAG29100.034.03058220
CACACGA30450.032.52211419
CACGAGA35750.027.6987721
CACACGT30250.026.72182819
CACGTAG26300.025.4113521
AAGAGCA140900.024.41730114
CGCCGTA119700.022.919647
CCGTATC125200.022.05427749
GCCGTAT132150.021.81942748
TCGCCGT129950.021.0039846
CGTATCA135050.020.73068850
GGTCGCC152100.019.53168344
GTCGCCG152500.019.4573445
GAGGGTA36850.018.4706571
GTGGTCG159750.018.33258242
ACACGTA36850.018.23222520
Produced by FastQC (version 0.11.2)