FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_rad27-dna2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_rad27-dna2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8914870
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2282042.5598129866167425No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC482930.5417128909339115Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG40000.051.26714718
AGAGCAC76750.046.2383715
GAGCACA76150.046.18724416
AGCACAC52750.042.061817
ACACGAG29300.041.32240720
CACACGA29350.040.89502719
CACGAGA35400.034.59533721
AAGAGCA140700.034.4532614
CACACGT27250.031.46183419
CAGATCG90100.029.8188826
CACGTAG22600.029.57179821
GCACAAG43200.027.46107118
CGAGATC46500.026.41204823
ACGAGAT47700.025.74759322
AAAGGGG86050.025.45918366
TAGATCG101650.025.1556936
GTGACTG26950.025.00251
AAGGGGG88150.024.81241267
AGGGGGG93350.024.67152268
CCGTATC159200.024.64091149
Produced by FastQC (version 0.11.2)