FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_rad27-dna2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_rad27-dna2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9935563
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2774402.792393344997158No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC502980.5062420720396016Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG55400.055.08022718
GAGCACA88150.049.9031816
AGAGCAC90350.049.31002415
AGCACAC68950.047.76021217
ACACGAG33050.046.47960320
CACACGA34200.045.02036719
CACACGT36450.041.66531419
CACGAGA37950.041.02924721
AAGAGCA158600.035.7495714
CACGTAG30650.035.61799221
ACACGTA36200.030.64220620
ACGAGAT53950.029.18544822
CGAGATC54200.028.792623
CAGATCG124850.027.131326
AAGGGGG91650.026.15612267
CCGTATC171750.025.34631749
GCACAAG40350.025.06359318
CGCCGTA178950.024.93121547
AAAGGGG98950.024.29756466
GCCGTAT190950.024.06079948
Produced by FastQC (version 0.11.2)