FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_rad27-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_rad27-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10293404
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3377143.2808777349067424No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC642820.6244970079868623Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG42100.051.62093718
AGAGCAC80750.045.55252515
GAGCACA79600.045.5055916
AGCACAC57950.040.94581217
ACACGAG31850.040.54285420
CACACGA32900.039.24969519
CACGAGA35850.035.52841621
AAGAGCA145450.032.94331414
CACACGT28350.031.35353519
CACGTAG26100.029.49482521
AAGGGGG107700.027.7816767
CCGTATC192050.027.46013349
CGCCGTA199600.027.03454847
CGTATCA199500.026.66258850
GCCGTAT213650.026.41937448
AGGGGGG120750.025.91335968
AAAGGGG116400.025.46604766
CAGATCG107750.025.45156
TCGCCGT211650.025.4457746
ACGAGAT50650.025.4234322
Produced by FastQC (version 0.11.2)