FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_rad27-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_rad27-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10231134
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3400593.323766456386946No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC374660.3661959661558533Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG41100.048.70426618
AGAGCAC67650.043.0949215
GAGCACA66750.042.626716
AGCACAC52300.040.55111717
ACACGAG27400.038.4415720
CACACGA29350.036.12687319
CACACGT29150.032.4131319
CACGAGA32950.031.96439721
CACGTAG23950.031.11928421
AAGAGCA131950.027.26816614
CAGATCG96800.025.4380346
CGCCGTA137700.024.06549847
ACACGTA30500.023.63483620
CCGTATC138350.023.49876849
CGAGATC46550.023.3016623
ACGAGAT45900.023.17470722
GCCGTAT148700.022.75591748
TCGCCGT145800.022.63252446
TAGATCG109600.021.8919096
CGTATCA150000.021.86033650
Produced by FastQC (version 0.11.2)