FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_exoi-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_exoi-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11479865
Sequences flagged as poor quality0
Sequence length76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6221075.419114249165822No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC706500.6154253556117603Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG49850.049.49378618
AGAGCAC92700.045.15704315
GAGCACA92350.044.98493216
AGCACAC67700.040.68493717
ACACGAG35700.040.3863820
CACACGA37450.038.6865619
CACGAGA41200.035.5012321
AAGAGCA171050.033.10975614
CACACGT33300.030.4765219
CACGTAG28000.029.86786521
GCACAAG55050.024.09398518
ACGAGAT62050.023.91038122
ACACGTA35150.023.59548620
AAGGGGG122850.023.41484367
CAGATCG182800.023.338286
AAAGGGG128950.022.25389366
CCGTATC264850.022.10519449
AGGGGGG141450.022.0706568
CGCCGTA272850.021.94395647
GAGAGAT102700.021.4695716
Produced by FastQC (version 0.11.2)