FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_exoi-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_exoi-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11546679
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4488053.8868751785686606No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC339900.294370355320348Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG33900.046.24944718
AGAGCAC58800.037.73790415
GAGCACA60700.036.38287716
AGCACAC45400.036.07770517
CACACGA27450.034.93213719
ACACGAG28800.032.92943620
CACGAGA34300.027.44349121
CACACGT25500.024.70297419
CGCCGTA122900.024.0306847
CCGTATC125450.022.9291549
GCCGTAT133150.022.73247348
TCGCCGT132400.022.12151546
AAGAGCA129950.021.817214
CACGTAG22850.021.59305421
GGTCGCC147700.020.89565844
GTCGCCG147100.020.79058845
CGTATCA140450.020.57998850
AAGGGGG75750.019.4033267
GTGGTCG158050.019.39395142
CTCGGTG144300.018.96289638
Produced by FastQC (version 0.11.2)