FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_dna2-exoi-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_dna2-exoi-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8686001
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3135163.60944006338475No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC404630.465841530527109Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG40950.051.44777718
AGAGCAC72050.047.55681615
GAGCACA73650.046.2847316
AGCACAC52300.043.96531717
ACACGAG26550.041.3865420
CACACGA27350.040.9441319
CACACGT29000.035.1151119
CACGAGA31700.033.8885621
AAGAGCA132200.033.25425714
CACGTAG24700.032.15902721
CAGATCG88250.031.6319076
ACACGTA27800.028.07075320
TAGATCG91700.027.3097956
CGAGATC41200.026.75360723
ACGAGAT41850.026.08754222
GCACAAG38850.025.85323518
CGCCGTA141900.023.87093747
AGAGATC70150.023.84771317
CCGTATC142300.023.46105449
GCCGTAT153000.022.91660748
Produced by FastQC (version 0.11.2)