FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_dna2-exoi-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_dna2-exoi-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10105175
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3867793.827533912079702No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC301030.297896869673212Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG26800.044.26584218
GAGCACA52600.035.26286716
AGAGCAC55500.034.1781715
AGCACAC39300.032.68094317
CACACGA21200.031.85770819
ACACGAG21900.030.51909320
CACGAGA28250.024.77264821
CACACGT22100.023.59321419
AAGAGCA117700.021.38085214
CGCCGTA114350.021.32952147
CACGTAG19150.021.19581221
CCGTATC116650.020.58010349
GCCGTAT124400.020.36575548
TCGCCGT121650.019.93451146
GGTCGCC133600.019.30334944
CGTATCA127450.019.0009150
GTCGCCG134650.018.91846545
AAAGGGG70800.018.19030266
GTGGTCG140850.018.1348542
CTCGGTG130250.017.81143238
Produced by FastQC (version 0.11.2)