FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_dna2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_dna2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8814911
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3284683.7262769868011145No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC385570.43740657166022434Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG38850.050.9829918
GAGCACA73800.047.0882516
AGAGCAC74950.046.92872215
AGCACAC51450.041.89937617
ACACGAG29100.041.00522220
CACACGA31600.038.09377319
CACGAGA33750.035.1474521
AAGAGCA139550.034.96324514
CACGTAG21550.031.50085621
CACACGT24950.031.41667219
CGCCGTA114150.029.4594947
CCGTATC115900.028.80463849
GCCGTAT131600.028.05251948
TCGCCGT122350.027.77109346
CAGATCG112400.027.2976176
CGTATCA127100.027.28509950
GCACAAG46500.027.16778218
ACACGTA25950.025.62087620
ACGAGAT46450.025.46208622
AGAGATC82600.025.42046217
Produced by FastQC (version 0.11.2)