FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n02_dna2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n02_dna2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8804690
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3583254.069706031671757No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC521710.5925364777181252Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG44650.055.02038618
GAGCACA72300.049.80949816
AGAGCAC74250.048.9732715
AGCACAC55850.047.3722317
CACACGA29500.045.07722519
ACACGAG31000.042.5571820
CACACGT29150.038.8957419
CACGAGA35450.038.3969721
CACGTAG26200.037.66269321
AAGAGCA136100.033.35389314
ACACGTA28950.033.1203720
ACGAGAT48950.027.59295322
CGCCGTA166000.026.72987447
CGAGATC51750.026.64078323
CCGTATC165650.026.5766149
GCCGTAT178350.025.85997248
GCACAAG33900.025.29148518
TCGCCGT176100.025.1173346
CGTAGAT39950.025.0501523
CAGATCG116400.024.9740186
Produced by FastQC (version 0.11.2)