FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_wt-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_wt-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11403736
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG671360.5887193460108161TruSeq Adapter, Index 9 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG164600.028.3954658
CGTGTGC47600.028.3181321
TGCTTGA177550.026.7552559
ATGCCGG22300.026.68207670
GCTTGAA193550.025.64655960
ACGTGTG31500.025.563961
CCGTCTT210600.024.27859951
GCCGTCT210100.024.21977650
TGCCGTC226400.023.8363549
ATGCCGT240300.022.7488348
CTTCTGC217800.022.43991755
CGTCTTC227800.022.12544352
GTGTGCT60450.022.1167682
TCTGCTT216950.021.81788357
TATGCCG250400.021.80453347
GTCTTCT233300.021.0337453
GATCTCG253950.020.22120340
GTATGCC275050.020.02846746
TTCTGCT234600.019.95257856
ATCTCGT262350.019.64158841
Produced by FastQC (version 0.11.2)