FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_wt-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_wt-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11278287
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATG406520.3604448086841557TruSeq Adapter, Index 1 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG39400.026.5711331
CGTGTGC55700.023.7613811
CTGCTTG114450.023.39986258
GCCGTCT140950.021.40377850
TGCCGTC157750.021.18762449
CCGTCTT146900.020.79873351
TGCTTGA130850.020.70582459
ATGCCGT170200.019.94612548
TATGCCG176350.019.58955847
GTGTGCT67450.019.5621812
GCTTGAA147600.019.44657760
CACGATC176200.018.9887537
GATCTCG171800.018.96797640
GACGTGT26750.018.7174781
CGATCTC180200.018.2003339
CGTCTTC164000.018.18427752
ACGATCT184600.017.88068238
ATCTCGT182050.017.84384241
GTATGCC196850.017.72714646
CTCGTAT194600.017.14277643
Produced by FastQC (version 0.11.2)