FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_triple-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_triple-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10119071
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATG1207961.1937459476270105TruSeq Adapter, Index 3 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG56100.037.0723271
CGTGTGC105000.030.744531
GCTTGAA265400.028.89836360
CTGCTTG243750.028.73908258
TGCTTGA249850.028.44137659
AAAAGGG142750.028.29487870
GTGTGCT118000.027.4656522
GCGTGAA64250.026.36962960
CTTGAAA307250.025.66853761
ATGCCGG58250.025.5971670
CTTCTGC291800.025.50597855
TCTGCTT282150.025.21226957
CCGTCTT345550.024.06451851
TTCTGCT276850.024.00036256
CTGCGTG48000.023.9952458
GCCGTCT341050.023.92037850
GTCTTCT311900.023.85779653
TGCGTGA49900.023.71101259
CGTCTTC339750.023.48863652
TGCCGTC412100.022.84500949
Produced by FastQC (version 0.11.2)