FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_triple-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_triple-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11343350
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG46500.025.5252461
CGTGTGC66850.025.139871
GACGTGT27800.022.2921371
GTGTGCT77950.021.5968952
GCGGGGT48550.018.30948851
TGTGCTC98200.017.3213373
GTGCTCT102550.016.6549994
AATCGCG41500.016.36332340
CGGGGTG60650.016.10107652
GCGGCGT20100.016.0185651
TCGGGGA23200.015.69249442
CGGCGTG19650.015.67478752
GTGTGGG33600.015.62899956
GGGTGTG35550.015.55603854
TGCGGGG28250.015.23786550
ATCGCGG29550.015.16355441
CGGGGAT23550.014.71603843
AATCGGG194200.014.65410240
ATCGGGG176100.014.59100641
TGAATCG250100.014.49973638