FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_rad27-exoi-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_rad27-exoi-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7761402
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAATCTCGTATG302000.3891049580990651TruSeq Adapter, Index 15 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG33450.031.9248311
CGTGTGC62000.028.8007221
GTGTGCT70050.025.6307072
CTGCTTG69850.021.8523158
GCCGTCT85550.020.82287850
ATGCCGG28350.020.24796570
GCGTGAA26050.019.4837860
GTGCTCT94150.019.2926924
GACGTGT19650.019.243591
CCGTCTT95050.019.18328751
TGTGCTC93950.019.1475013
TGCTTGA83500.019.03252259
GCTTGAA91150.018.89388860
TGCCGTC109800.018.80571649
ATGCCGT116950.018.61359848
TATGCCG132000.017.68686547
CTTCTGC95850.017.02001455
CGTCTTC104900.016.48353852
TCTGCTT99400.015.91939557
GTATGCC152800.015.82900746
Produced by FastQC (version 0.11.2)