FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_rad27-exoi-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_rad27-exoi-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11785366
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATG344660.29244743014345076TruSeq Adapter, Index 6 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC47000.023.3915311
ACGTGTG33550.021.7068941
GCCGTCT87150.021.40409550
TGCCGTC109150.020.29613749
TATGCCG121900.019.86876347
ATGCCGT116150.019.85635848
GTGTGCT60900.018.332882
CCGTCTT107400.018.02005451
GTATGCC138350.017.80991746
CTGCTTG86900.017.72604658
CGTATGC141200.017.10602645
TATCTCG169750.017.0943240
CTCGTAT150850.016.94031543
ATCTCGT175550.016.07223741
TCGTATG151200.016.02138544
TCTCGTA164950.015.95907242
GTCACGC219300.015.32025330
TCACGCC220450.015.240334531
TGTGCTC74200.015.2352783
TGCTTGA106750.015.1826659
Produced by FastQC (version 0.11.2)