FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_rad27-dna2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_rad27-dna2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8914870
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCATCTCGTATG144210.16176343569788454TruSeq Adapter, Index 14 (97% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC52000.025.4503841
ACGTGTG31850.024.5132731
TATGCCG80350.023.73996547
GCCGTCT49550.023.2372750
GTGTGCT57700.023.1703532
TGCCGTC67750.022.88337149
GCGTGAA37400.022.7431460
CATCTCG60650.022.68077940
GTATGCC89000.022.17984646
ATGCCGT73150.022.15094648
CGTATGC87850.022.114645
TCGTATG82950.020.29872544
CTCGTAT62150.019.7136543
TGCGTGA30750.019.12501559
CTGCGTG28250.018.58892858
CCGTCTT64850.018.51045251
ATCTCGT71600.018.28515641
TGCCGGC28350.018.0229349
CATGTCG53900.017.9881840
TCTCGTA70650.017.29232442
Produced by FastQC (version 0.11.2)