FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_rad27-dna2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_rad27-dna2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9935563
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG45050.029.6898841
GACGTGT25850.028.4445251
GCGTGAA37700.023.76952660
TATGCCG62800.023.68626247
CGTGTGC72700.023.1659771
GCCGGCG20750.022.938150
GTATGCC71000.022.18327946
CCGGCGT23350.021.73290351
GTGTGCT80300.021.183132
ATGCCGT56050.021.04208248
TGCGTGA32750.020.94937359
TGCCGTC51500.020.93052549
TGCCGGC31500.020.88737749
CTGCGTG26750.020.80841358
GTGCGTG17800.020.2573658
ATGCCGG37750.020.1176548
CGTATGC76700.019.7614545
GCCGTCT36350.019.35210850
TGTGCTC98300.017.3393943
CAGTGAT287400.017.15785836
Produced by FastQC (version 0.11.2)