FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_rad27-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_rad27-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10293404
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG34700.027.4428121
CGTGTGC56550.025.382851
TATCGCG50950.023.77075640
ATCGCGG34850.022.80129441
GCCGGCG16300.022.3306850
GCGGGGT47700.021.49805351
GTGTGCT67150.021.4213982
TCGGGGA29650.020.66092342
CGGGGTG59200.020.57556352
CGGCGTG28150.020.2676552
GCTTATC303700.020.21239737
CCGGCGT19150.019.92083751
CGGGGAT28950.019.70968443
GCGGCGT23150.019.65360551
ATCGGGT42950.019.479241
TAGCTTA334900.019.41626535
ACTAGCT337600.019.29217533
CACTAGC340050.019.23551432
CTTATCG268050.019.20886238
TGCCGGG25550.019.17737449
Produced by FastQC (version 0.11.2)