FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_rad27-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_rad27-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10231134
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG38450.026.587841
CGTGTGC56700.022.0435581
GACGTGT24550.021.3912561
TATCGCG54000.019.57584640
GTGTGCT65050.019.530512
ATCGCGG31950.017.63956541
CCGGCGT20150.017.5424451
GCCGGCG17150.017.34613450
GTGCGTG15850.016.78647858
GCGTGAA30650.016.67459160
GTATCGC66100.016.57479339
GTGCTCT78200.016.425154
TCGCGGA17500.016.40236542
TATGCCG34300.016.3266547
TGTGCTC79300.016.1531773
TGCCGGC25950.016.04927449
GCCGGGG19250.015.99925850
CCGATGT244250.015.96217734
CTGCGTG22650.015.92004558
CGATGTA245950.015.83761835
Produced by FastQC (version 0.11.2)