FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_exoi-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_exoi-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11479865
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATG156780.13656955025167977TruSeq Adapter, Index 18 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG39250.025.689921
CGTGTGC68400.023.9551931
GCGTGAA48350.022.15426360
GTGTGCT77900.021.2961622
CATCTCG101100.020.1497740
CGCATCT140950.019.54392238
TATGCCG77750.019.35800747
CTGCGTG34250.019.01309658
TGCGTGA39850.018.88668459
CCGGCGT32300.018.85309251
GCCGGCG28750.018.6250350
GTATGCC90300.018.60554146
GCATCTC115750.018.4159739
CCGCATC367700.018.09325837
CGTATGC84050.018.03399745
GCCGTCT44200.017.89491350
TGCCGGC43000.017.8245749
GTGCGTG27550.017.79126758
CATCGCG114750.017.72236640
TCCGCAT388000.017.64286836
Produced by FastQC (version 0.11.2)