FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_exoi-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_exoi-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11546679
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATG361530.3131030142952792TruSeq Adapter, Index 4 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC43650.024.3032041
TATGCCG127100.022.30564747
TGCCGTC118500.021.91446149
GCCGTCT103200.021.9078650
ATGCCGT126750.021.0954548
CTGCTTG94950.020.83161458
CCGTCTT113150.020.56896251
GTATGCC147550.019.71203846
GTGTGCT55750.018.896362
ACGTGTG30950.018.8912621
CGTATGC158150.018.26058445
CTCGTAT157600.018.191243
AATCTCG165250.017.94079840
ATCTCGT165100.017.68286541
TGCTTGA115100.017.51769859
TCTCGTA166800.017.39771342
TCGTATG170600.017.05130244
GCTTGAA129950.016.97040760
ACTGACC202700.016.90311433
GCGTGAA32800.016.32847460
Produced by FastQC (version 0.11.2)