FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_dna2-exoi-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_dna2-exoi-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8686001
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG36500.027.2425441
GACGTGT24450.023.6280161
CGTGTGC56300.022.0149171
TATGCCG39650.021.80323247
GCGTGAA25000.020.0228460
GTATGCC48300.019.4202546
CATCTCG40650.019.37477340
GTCCATC109200.019.35726437
TGCCGGC20950.018.70967149
CGTCCAT188700.018.6391636
CGTATGC45750.018.5932345
GCCGGCG14800.018.44452750
GTGTGCT67850.018.2605172
ATGCCGT38400.017.68072948
CCGTCCA224600.017.48287235
CCGGCGT16550.017.34005551
CATCGCG36600.016.92804740
TGCCGTC36000.016.81805449
ACCCGTC240350.016.73037133
TCACCCG241650.016.59663231
Produced by FastQC (version 0.11.2)