FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_dna2-exoi-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_dna2-exoi-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10105175
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTGC38450.021.304831
ACGTGTG28600.019.7069071
CATCGCG35050.018.97431840
GTGTGCT48100.017.2443942
ATCGCGG23000.016.43723541
GACGTGT21400.015.7042091
ATCGGGG58600.015.29238341
GCGGGGT29000.015.20600451
CATCGGG73050.015.04561540
CCCCGGG14300.014.6839447
TGCGGGG16700.014.46022850
CAGATCA197050.014.4574135
AGTCACC203550.014.25329329
TGTGCTC59800.014.22164253
CAGTCAC205700.014.1213328
CGGGGTG35600.013.961513552
GATCATC172400.013.90565837
TCCAGTC212950.013.85420826
CCAGTCA209950.013.85214127
AGATCAT198550.013.80175836