FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_dna2-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_dna2-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8814911
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG651450.7390318518247093TruSeq Adapter, Index 11 (100% over 49bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG28100.028.4076981
GCGTGAA36150.028.17692860
CGTGTGC50950.027.4867461
CTGCTTG114550.027.1698458
GCCGTCT145050.025.86497750
GCTTGAA135600.025.34899560
CCGTCTT152800.025.12556351
TGCCGTC177300.025.02925949
TGCTTGA128150.024.72043859
ATGCCGT189250.024.11455248
TGCGTGA30800.023.86683359
TATGCCG209300.022.91016847
GTGTGCT60650.022.9087892
CATCTCG222750.022.6111440
CTTCTGC148100.022.5749255
CGTCTTC161550.022.42512752
CTGCGTG28250.022.05880458
GTATGCC226800.021.63624446
ACATCTC239650.021.36713439
ATCTCGT233550.021.2677441
Produced by FastQC (version 0.11.2)