FastQCFastQC Report
Tue 19 Jun 2018
H3YTNAFXY_n01_dna2-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YTNAFXY_n01_dna2-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8804690
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTGTG37900.031.8698141
CGTGTGC62550.026.4188751
GACGTGT20150.025.3674891
GTGTGCT69950.023.8165172
CATCTCG50950.023.0830740
AGGCATC176950.022.44835737
CATCGCG54550.022.39387140
CCGGCGT17400.021.72331851
GCGTGAA31550.021.63495360
TATGCCG40850.021.50579547
GCATCTC57550.021.1047539
GCCGGCG13950.021.0747150
CCGGGTG16250.020.89317952
GGCATCT72850.020.6118238
GGCATCG120500.020.47814838
CTGCGTG19450.020.15840158
ATGCCGG23900.019.91533948
GTATGCC48700.019.90797646
ATCGCGT32450.019.74067541
GTGCGTG15650.019.68457458
Produced by FastQC (version 0.11.2)