FastQCFastQC Report
Fri 24 Jun 2022
H3YJ2BGXM_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2BGXM_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385122071
Sequences flagged as poor quality0
Sequence length10
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACTCTCCCG21817248956.65021701651578No Hit
AACGAACTGT13107229134.03395984542262No Hit
AGATCTCGGT194171205.0418091982061455Illumina Single End PCR Primer 1 (100% over 10bp)
TACTCTCCCG12409400.32221991244952564No Hit
GGGGGGGGGG11339200.2944313207123359No Hit
GACTCTCCGG4359030.11318567094016276No Hit
GCCTCTCCCG4306240.11181493672430942No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)