FastQCFastQC Report
Fri 24 Jun 2022
H3YJ2BGXM_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2BGXM_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences385122071
Sequences flagged as poor quality0
Sequence length28
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCCCCTACACGACGCTCTTCCGATC20684760.5370961977403782Illumina Single End PCR Primer 1 (95% over 24bp)
TTGCTCTCTACACGACGCTCTTCCGATC13100990.3401775952747201Illumina Single End PCR Primer 1 (100% over 21bp)
GTCCTCCCTACACGACGCTCTTCCGATC7199110.18693060050562516Illumina Single End PCR Primer 1 (100% over 24bp)
ATGGCCCCTACACGACGCTCTTCCGATC7007360.18195165968558577Illumina Single End PCR Primer 1 (95% over 24bp)
GGTTGCTCTACACGACGCTCTTCCGATC5615480.14581039163553938Illumina Single End PCR Primer 1 (100% over 21bp)
GTGCCTTCTACACGACGCTCTTCCGATC5582140.14494469209478258Illumina Single End PCR Primer 1 (95% over 22bp)
ATCACCCCTACACGACGCTCTTCCGATC5362690.1392464988068679Illumina Single End PCR Primer 1 (95% over 24bp)
AACCGCTCTACACGACGCTCTTCCGATC5258100.13653073651029468Illumina Single End PCR Primer 1 (100% over 21bp)
GTTCCTTCTACACGACGCTCTTCCGATC4907780.12743439988408248Illumina Single End PCR Primer 1 (100% over 21bp)
CTAACCCCTACACGACGCTCTTCCGATC4799350.12461892894214313Illumina Single End PCR Primer 1 (95% over 24bp)
GTCCTCCTACACGACGCTCTTCCGATCT4589420.1191679300041986Illumina Single End PCR Primer 1 (100% over 23bp)
GGTGCTTCTACACGACGCTCTTCCGATC4536560.11779537818283076Illumina Single End PCR Primer 1 (100% over 21bp)
CAATCCCCTACACGACGCTCTTCCGATC4412360.11457042668427073Illumina Single End PCR Primer 1 (100% over 23bp)
GTACACCCTACACGACGCTCTTCCGATC4410520.11452264962503278Illumina Single End PCR Primer 1 (95% over 24bp)
TGTGGCTCTACACGACGCTCTTCCGATC4390140.11399346676238661Illumina Single End PCR Primer 1 (100% over 21bp)
GGTTGATCCTGCCAGTAGTTATATGCTT4352340.11301195978456399No Hit
ACGCCTTCTACACGACGCTCTTCCGATC4315260.11204914817774753Illumina Single End PCR Primer 1 (100% over 21bp)
TAACCTATTCTCAAACTTTAAATGGGTA4118210.10693258865446847No Hit
CTGCTCCCTACACGACGCTCTTCCGATC4095570.10634472309949745Illumina Single End PCR Primer 1 (100% over 24bp)
ACCACATCTAAGGAAGGCAGCAGGCGCG4087880.10614504615083462No Hit
CGTAACCCTACACGACGCTCTTCCGATC4059040.10539619267886623Illumina Single End PCR Primer 1 (100% over 23bp)
AAAGGCTCTACACGACGCTCTTCCGATC4032850.10471614855851769Illumina Single End PCR Primer 1 (100% over 21bp)
ACGTCCTCTACACGACGCTCTTCCGATC3966460.10299227955699272Illumina Single End PCR Primer 1 (100% over 21bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)