FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n02_exp269-6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n02_exp269-6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10344573
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1300771.2574419456462824Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG735530.7110298317774935No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC115650.045.0917815
GAGCACA113200.045.0154116
ACACGAG39450.044.6245220
CACACGA40400.043.92106219
GATCGAG38900.043.8176088
GCACACG61900.043.5355718
AGATAGA139150.040.7982147
AGCACAC76800.040.2386717
GATAGAT144050.039.4100468
CACGAGA45500.038.38287421
AAGAGCA193150.037.45437214
ATAGATC157750.035.898689
GCACAAG52250.035.2994918
AGATCGA53450.033.2651567
CAGATAG59450.032.6749276
GCAGATC81400.032.1205025
ATCGAGA54800.031.7427949
CACGTAG26500.031.69667421
CGGGGAC8900.030.6971071
TCGAGAT59250.029.41752610
Produced by FastQC (version 0.11.2)