FastQCFastQC Report
Sat 11 Aug 2018
H3YJ2AFXY_n02_exp269-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3YJ2AFXY_n02_exp269-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9190862
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG746500.8122197896127697No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC590420.6423989392942686Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCAC82650.041.0758515
GAGCACA81300.040.37880316
AGATAGA109950.037.9132277
GATAGAT112250.037.385438
GATCGAG32150.037.3407178
GCACACG38000.036.4720218
ACACGAG27600.035.88682620
ATAGATC115350.035.7734649
AGCACAC49150.034.89140317
CACACGA28300.034.87473319
AAGAGCA154950.033.49710514
CGGGGAC9000.031.5267011
CACGAGA33050.031.23979821
CAGATAG49200.030.7321476
AGATCGA42300.029.373947
GCACAAG50750.028.48145918
ATCGAGA44250.027.2088369
GAGCAAG56650.026.5032116
GCAGATC68450.026.231345
TCGAGAT46350.026.20260610
Produced by FastQC (version 0.11.2)